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Since the first edition of our book Tissue Culture in Fores- try in 1982 we have witnessed remarkable advances in cell and tissue culture technologies with woody perennials. In addition to forest biologists in government, industry, and universities, we now have molecular biologists, genetic engineers, and biochemists using cell and tissue cultures of woody species routinely. There- fore, the time has come for an update of the earlier edition. In our present effort to cover new developments we have expanded to three volumes: 1. General principles and Biotechnology 2. Specific Principles and Methods: Growth and Development 3. Case Histories: Gymnosperms, Angiosperms and Palms The scientific barriers to progress in tree improvement are not so much lack of foreign gene expression in plants but our current inabili ty to regenerate plants in true-to-type fashion on a mas- sive and economic scale. To achieve this in the form of an appro- pr iate biotechnology, cell and tissue culture will increasing ly require a better understanding of basic principles in chemistry and physics that determine structural and functional relationships among molecules and macromolecules (proteins, RNA, DNA) within cells and tissues. These principles and their relationship with the culture medium and its physical environment, principles of clonal propagation, and genetic variation and ultrastructure are discussed in volume one.
