This Pharmacology Practical Lab Manual, aligned with the PCI syllabus for B. Pharm Second Year, addresses the pressing need for discovering new agents to counteract both modified and emerging adverse effects. The quest for efficient methods to evaluate test drugs swiftly, affordably, and effectively is paramount. These methods aid in discovering and refining drugs sourced from natural, synthetic, and semi-synthetic pathways. The manual aims to develop laboratory test protocols for the pharmacological assessment of crude and formulated drugs, emphasizing simplicity, time efficiency, and cost-effectiveness. It serves as a valuable resource for students in this field.