The search for new antibacterial agents directed towards novel targets became highly imperative. Mycobacterium tuberculosis (Mtb), causative organism of tuberculosis (TB), is a successful pathogen that overcomes the numerous challenges by the immune system of the host. In the last 40 years few drugs have been developed, while the drug resistance problem is increasing, there is thus a pressing need to develop new anti-TB drugs active against both the acute and chronic growth phases of the Mycobacterium tuberculosis. Hence an attempt was made in the present study to establish the structural, functional characterization of Mtb-MenF at molecular level using in silico techniques.