Prokaryotic Genomics

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This text provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and new tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology. It features: specific, step-by-step protocols for prokaryotic genomics and proteomics; genome mapping and DNA fingerprinting of bacteria; bacterial genetics, including methods to identify exchange and transfer genes; expression measures, genetic footprinting and microbial two-hybrid assay; protein purification with inteins; tips for data interpretation and useful websites; a troubleshooting guide; a guide to solutions; and a guide to protocols. Protocols are accompanied by notes and lab tricks and alternative methods are provided and discussed. The text is intended for: researchers working in microbiology, molecular and cell biology, biotechnology and biochemistry; biotechnology companies; and graduate and postgraduate students.