Methods for Affinity-Based Separations of Enzymes and Proteins

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Extensive troubleshooting guide Big concerns of biotechnology are either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90 percent of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors. As each chapter includes a succinct introduction, the volume is a handy and instructive reference also for students majoring in these disciplines. The special focus plus the most detailed protocols of up-to-date methods make this book a very useful and unique reference.