Innovative study is a significant factor for the student and researcher in the modern age of technologies. With view to the achieving this vision, data have been invented and put in this book as vital role. Transgenic plant production, T-DNA, DNA cloning and cell culture are discussed well having innovative data. GM (Genetically Modified) seedless fruit and vegetable was invented, plant growth hormone’s effect, plant regeneration, cell clone and genomics are discussed. Effects of gibberellic acid, cytokinin, auxin are shown in different crops. Plant hormone isolation like abscisic acid, cytokinine are described well. Auxin’s effects on GM (genetically modified)seedless fruit and vegetable production are stated detailed. Different methods of Genetic engineering and its application in crop production are noted well. Implementation of molecular plant biotechnology in fruit and ornamental plant related to biomedicine or herbal medicine are described. Innovative data are mentioned that abscisic acid and gibberellin affected the gene in GM crops and developed the plant growth and production as well as improved the fruit, flower and vegetable quality applying biotechnological approach.