The second edition of the highly successful grey book Drosophila: A Laboratory Handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. The original format is maintained, providing a handy reference guide to most aspects of the biology of Drosophila. Each of the thirty-six chapters summarizes the present state of the subject, written with a historical perspective, but with an emphasis on the practical use of genetic and other methods. Extensively referenced.