PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including:

*real time qRT-PCR

*rapid generation of gene targeting constructs

*PCR multiplexes

*PCR-based mutagenesis

*identification of microdeletions and microduplications

*DNA methylation analysis

*forensic genetic DNA typing

*genotyping

*identification of mutations in single cells

*whole genome amplification

*diagnosis of infectious diseases

*inverse PCR-based RFLP

This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.