Various naturally occurring phytochemicals exhibit anti-inflammatory activity and are considered to be potential drug candidates against inflammation-related pathological processes. Here, new flavonoid glycoside, kaempferol 3-O- -[(6-P coumaroyl galactopyranosyl-O- - (1 4)-O- -rhamnopyranosyl-(1 4)]-O- -rhamnopyranoside 1, in addition to five known flavonoid glycosides (2-6) kaempferol 3-O-[- rhamnopyranosyl-(1 4)-O- -rhamnopyranosyl-(1 6)-O]- - galactopyranoside (kaempferol 3-O- -isorhamninoside) 2, quercetin 3-O- [(2,3,4-triacetyl- -rhamnopyranosyl)-(1 6)- -galactopyranoside 3, quercetin 3-O-[(2,4-diacetyl- -rhamnopyranosyl)-(1 6)]-3,4-diacetyl- - galactopyranoside 4, quercetin 3-O-[(2,4-diacetyl- -rhamnopyranosyl)- (1 6)]-2,4-diacetyl- -galactopyranoside 5, quercetin 3-O-[(2,3,4-triacetyl- -rhamnopyranosyl)-(1 6)-3-acetyl- -galactopyranoside 6 were isolated from bell pepper (Capsicum annum L.) fruits and tested for both antiinflammatory activity through cytokine production (TNF- and IL-1 ) and antioxidant activity through scavenging effect on 1,1-diphenyl-2- picrylhydrazyl (DPPH) assay. Compounds 1-3 significantly suppressed production of TNF- / IL-1 in cultured THP-1 cells previously costimulated by LPS in a dose-dependent manner (10.2/49.1, 28.1/55.7, and 35.2/57.5 M respectively) whereas compounds 4-6 have relatively weaker inhibitory activity. (45.3/73.5, 48.2/65.6, and 42.2/67.4 M respectively). All compounds 1-6 showed no cytotoxic activity against the growth of THP-1where the percentage of cell viability was (127.4, 108.5, 105.4, 103.9, 103.4, and 104.2 M respectively). All isolated compounds exhibited higher radical scavenging activity than ascorbic acid in (DPPH) assay. These results indicated that bell pepper fruits could be an effective candidate for ameliorating inflammatory-associated complications.
