Genetic Recombination Research Progress

Genetic recombination is the process by which a strand of genetic material (usually DNA; but can also be RNA) is broken and then joined to a different DNA molecule. In eukaryotes recombination commonly occurs during meiosis as chromosomal crossover between paired chromosomes. This process leads to off-springs having different combinations of genes from their parents and can produce new chimeric alleles. In evolutionary biology this shuffling of genes is thought to have many advantages, including that of allowing sexually reproducing organisms to avoid Muller’s ratchet. However, a recombination pathway in DNA is any way by which a broken DNA molecule is reconnected to form a whole DNA strand. In molecular biology recombination can also refer to artificial and deliberate recombination of disparate pieces of DNA, often from different organisms, creating what is called recombinant DNA. Enzymes called recombinases catalyse natural recombination reactions. RecA, the recombinase found in E. coli, is responsible for the repair of DNA double strand breaks (DSBs). In yeast and other eukaryotic organisms there are two recombinases required for repairing DSBs. The RAD51 protein is required for mitotic and meiotic recombination and the DMC1 protein is specific to meiotic recombination. This book presents the latest research in the field.