This study examines the historical hydrology and ecology of the Petaluma River watershed prior to major Euro-American modification, and analyzes landscape changes over the past two centuries. Synthesizing information from hundreds of archival documents, the research examines the historical form and function of wetland, riparian, and aquatic habitats and stream channels throughout the watershed, providing insights into habitat extent and distribution, streamflow and sediment dynamics, vegetation composition, wildlife support, and landscape change. Findings from this research can be used to help set restoration targets and to prioritize multi-benefit opportunities to restore wildlife habitat, enhance flood protection, increase groundwater recharge, and improve sediment management.