For those new to this technique, this guide provides basic tips, key skills, awareness and guidance on good practice of High Performance Liquid Chromatography (HPLC). It will help build understanding of the important issues to consider during analysis and how to develop further skills. HPLC is one of the most widely used techniques in industry to separate and analyze compounds through the mass transfer of analytes between stationary and mobile phases. This guide offers an introduction to the technique, including a look at the basic theory, HPLC components, system parameters, suitability checks and testing. Later sections cover calibration, problem solving and data handling. High Performance Liquid Chromatography is one of the Practical Laboratory Skills Training Guides , a series that aims to make achieving best practice easy. These manuals will enable both experienced and inexperienced staff to get the essential basics of any experiment right simply by following the clear and easy to use instructions provided. The guides are written by experienced scientists and include minimal theory, plenty of practical exercises in order to assess competence, and trouble-shooting information.