The idea for an ACS symposium series for eye tracking started to form in the summer of 2015. Each of the editors had experience with eye tracking from our dissertation research and upon further discussion, discovered they all had the same questions: What type of eye tracker should be used? What types of research questions can eye tracking answer? What should be considered concerning experimental design? How could the results be analyzed in a meaningful way? With no clear help from the existing chemistry education research (CER) literature, the editors had to expand their reading to the fields of psychology, consumer analysis, and product design to find suitable answers. When they spoke with chemistry education researchers who were interested in eye tracking, they discovered these same questions were prevalent among them as well; and often, they did not even know where to start. At that point, they decided that an eye-tracking guidebook aimed at chemistry education researchers could be a valuable resource.This book is not intended to be a review of eye tracking in CER literature; rather, it is meant to be a tool to help answer the same questions the editors had when they started out in the field. The editors also hope that those with some experience in eye tracking will find among these pages ideas they had not considered applying to their research, but that will be valuable to them moving forward.