Hernando de Soto, the Spanish conquistador, is legendary in the United States today: counties, cars, caverns, shopping malls and bridges all bear his name. This work explains the historical importance of his expedition, a journey that began at Tampa Bay in 1539 and ended in Arkansas in 1543. De Soto’s explorations, the first European penetration of eastern North America, preceded a demographic disaster for the aboriginal peoples in the region. Old World diseases, perhaps introduced by the de Soto expedition and certainly by other Europeans in the 16th and 17th centuries, killed many thousands of Indians. By the middle of the 18th century only a few remained alive. The de Soto narratives provide the first European account of many of these Indian societies as they were at the time of European contact. This work interprets these and other 16th-century accounts in the light of new archaeological information, resulting in a more comprehensive view of the native peoples. Matching de Soto’s camps to sites where artifacts from the de Soto era have been found, the authors reconstruct his route in Florida and at the same time clarify questions about the social geography and political relationships of the Florida Indians. They link names once known only from documents (for example, the Uzita, who occupied territory at the de Soto landing site, and the Aguacaleyquen of north peninsular Florida) to actual archaeological remains and sites.