Clamp Loader of Escherichia Coli Dna Polymerase III: Kinetics of the Atp-dependent Steps in the Sliding-clamp Loading Reaction

Abstract: Sliding clamps tether DNA polymerases to DNA to increase the processivity of synthesis. The Escherichia coli
complex loads the
sliding clamp onto DNA in an ATP-dependent reaction in which ATP binding and hydrolysis modulate the affinity of the
complex for
and DNA. This dissertation covers research describing the kinetic mechanism of clamp loading by gamma complex.

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