The Proteome Revisited: Theory and Practice of all Relevant Electrophoretic Steps

Preface. Part I: Isoelectric Focusing: Fundamentals. Perspective s and Limits. Optimization of the Separation Process. P art I.I: Isoelectric Focusing: Fundamentals. Introduction. Isoe lectric Focusing: Principles and Historical Aspects. References. Electr olyte Dissociation in Water Solution. Simple Electrolytes. Stepwise and parallel dissociation schemes for a bivalent prot olyte. Relative concentration of different protolyte forms
for stepwise and parallel schemes.
Hydrogen ions concentra tion and buffer capacity. Ionization coefficient.
Isoelectric point. Mobility of protolyte molecule. Non additive sum for buffer capacity in case of stepwise dissociation.
Non amphoteric compounds and buffer capacity in ‘isoprotic s tate’. References. Dissociation of Polyvalent Elec trolytes. Acid-base equilibria, macroscopic and microscopic constants. Dissociation schemes of a hybrid type.
Proton transfer tautomerism. Schemes with independent dissoci