7 ½ X 9 ¼ in Contributors. Preface. Purification and Recons titution of Drosophilia g- - Tubulin Complexes. Us ing Rapid Freeze and Freeze-Substitution for the Preparation of Yeast Ce lls for Electron Microscopy and Three-Dimensional Analysis. Digital Fluorescence Microscopy of Cell Cytoplasts with and without the Centrosome. Methods for the Study of Pericentrin in Centro some Assembly and Function. Molecular Dissection of Yeast S pindle Pole Bodies by Two Hybrid in Vitro Binding, and Co-purif ication. Genetic Analysis of Yeast Spindle Pole Bodies. Methods for the Study of Centrosomes in Drosophilia d uring Embryogenesis. Methods for Identification of Centroso me-Associated Proteins. Reconstitution of Centrosome Microt ubule Nucleation in Drosophilia. Reconstitution of Centrosome Microtubule Nucleation in Spisula. In Vitro Approaches for the Study of Microtubule Nucleation at the Fission Yeast Spindle Pole Body. Gamma Tubulin and Microtu