Immunology of Infection

9 11/16 X 7 7/16 in Contributors Preface to the 2nd edition Prefac e to the 1st edition

Introduction The immune response to infectious agents

General Methods Phenotyping and separation of leukocyte populations based on affinity labelling. Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells. Measurement of cellular prol iferation. Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design. The use of tetramers in the quantitative analysis of T cell respons es. Using microarrays for studying the host transcriptional response to microbial infection. Genome-wide expression pr ofiling of intracelllular bacteria: the interaction of Mycobacterium tub erculosis with macrophages.

Murine Mo dels Management of immuno-compromised and infected animals. In Vitro Analysis Isolation