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Table of Contents
Chapter 1 Over production and purification of RFC-related clamp loaders and PCNA-relate d clamps from Saccharomyces cerevisiae Chapter 2Functional Assays for Replication Protein A (RPA) Chapter 3 Human DNA Ligases I, III, and IV
purificatio n and new specific assays for these enzymes.
Chapter 4 Enzymatic Mechanism of the WRN Helicase/Nuclease
Chapter 5 Analysis of the DNA Unwinding Activity of RECQ Fa mily Helicases Chapter 6 Characterization of C heckpoints Responses to DNA damage in Saccharomyces cerevisiae: Basic Protocols Chapter 7 Recruitment of ATR- ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage Chapter 8Multiple approaches to study S. cerevisiae Rad9, a prototypical checkpoint protein Chapter 9 Meth ods for studying adaptation to the DNA damage checkpoint in yeast Chapter 10 DNA damage-induced phosphorylation of Rad
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Table of Contents
Chapter 1 Over production and purification of RFC-related clamp loaders and PCNA-relate d clamps from Saccharomyces cerevisiae Chapter 2Functional Assays for Replication Protein A (RPA) Chapter 3 Human DNA Ligases I, III, and IV
purificatio n and new specific assays for these enzymes.
Chapter 4 Enzymatic Mechanism of the WRN Helicase/Nuclease
Chapter 5 Analysis of the DNA Unwinding Activity of RECQ Fa mily Helicases Chapter 6 Characterization of C heckpoints Responses to DNA damage in Saccharomyces cerevisiae: Basic Protocols Chapter 7 Recruitment of ATR- ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage Chapter 8Multiple approaches to study S. cerevisiae Rad9, a prototypical checkpoint protein Chapter 9 Meth ods for studying adaptation to the DNA damage checkpoint in yeast Chapter 10 DNA damage-induced phosphorylation of Rad