Regulators and Effectors of Small GTPases: Ras Family

Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hyd rolysis;
Schwann cell preparation from single mouse embryo s: Analyses of neurofibromin function in Schwann cells; Regulation of th e nucleotide state of oncogenic Ras proteins by nucleoside diphosphate k inase; Measurements of TSC2 GAP activity toward Rheb; Characterization o f AND-34 function and signalling; Studying the spatial and temporal regu lation of Ras GTPase-activating proteins; Activation of Ras Proteins by Ras Guanine Nucleotide Releasing Protein Family Members; Ras and Rap1 Ac tivation of PLC? lipase activity; Specificity and expression of RalGPS as RalGEFs; Biochemical and biological analyses of Rgr RalGEF oncogene; Analysis of Ras Activation in Living Cells with GFP-RBD; Genetic and pha rmacologic analyses of the role of Icmt in Ras membrane association and function; Characterization of the activation of the Rap specific exchang e factor epac by cyclic nucleotides; Biochemistry of the Rap-specific gu anine nucleotide exchange factors PDZ-GEF1 and 2; Characterization of in teractions between Ras family GTPases and their effectors; Genetic and p