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Details methods for the study of RNA processing which pertain to preparation of substrates and processing enzymes or factors, and to analysis of the resulting products. Includes methods for the…
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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently…
This volume addresses a variety of areas in which computers are used to manage and manipulate nucleic acid and protein sequence data. The manipulations include searching, aligning, and determining the…
The aim of this volume is to give recombinant DNA researchers current knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells.
This volume is the first comprehensive compilation of methods for the assay and purification of enzymes involved in the utilization of reduced one-carbon compounds and higher hydrocarbons. In the last…
This text concerns the computer-based design and modelling, computational approaches and instrumental methods for elucidating molecular mechanisms of protein folding.
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This text draws together the methodology for in vivo genetic engineering and for genetic analysis in a wide range of bacteria. The bacterium Escherichia coli is covered, along with other…
This book is aimed at biochemists, geneticists, molecular biologists, cell biologists, microbiologists, genetic engineers, analyticalchemists, biophysicists, and industrial researchers working on protein products.
A respected publication in the field of biochemistry since 1955, this series presents new ideas on classification of proteolytic enzymes, covers 100 individual proteolytic enzymes, offers numerous medical implications of…
This volume provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors.
Results indicate that redox-active prosthetic groups can arise from pre-existing amino acid side chains in proteins. In this volume, methods are described for the detection and characterization of such prosthetic…
Useful for researchers in various fields of life sciences, this title includes features such as cell cycle regulators in mammalian systems, cell cycle control in yeast and fungal systems, and…
Features the description of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. This volume discusses some of the attempts to apply techniques of molecular…
In this volume, the major topics covered include: Theory and Practical Consideration, General Techniques, Measurement of Subcellular Relations and Volume Determinations, Imaging of Processes, Imaging of Ions, Imaging of Specialized…
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. This volume is an overview…
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This is a reproduction of a book published before 1923. This book may have occasional imperfectionssuch as missing or blurred pages, poor pictures, errant marks, etc. that were either part…
This volume in a series on methods in enzymology is concerned with recombinant DNA. It discusses strategy for producing single-stranded DNA in polymerase chain reaction, reverse cloning procedure for rapid…
The subject of biomembranes has been divided into five topics for this series. This and the subsequent volume discuss the topic announced in the subtitle. Annotation copyright Book News, Inc…
FROM THE PREFACE: Volumes 96 to 98, Parts J, K, and L of the Biomembranes series, focus on methodology to study membrane biogenesis, assembly, targeting, and recycling. This field is…
This volume and its companion Volume 284 consolidate methods for purifying, characterizing, isolating, and applying lipases, and covers its uses in drug design and as additives in detergent development. The…
Recent advances in understanding the biological role of singlet oxygen in the pathways of cellular responses to ultraviolet-A radiation: its key position in photodynamical effects, and its generation by photochemical…
This volume covers cellulose and hemicellulose and includes proven and reproducible methods for research related to the conversion of carbohydrate polymers to usable monomeric units. Sections on the preparation of…
This volume describes methods for the assay, purification and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins and guanylyl cyclases. Topics covered are the determination of activity inadenylyl cyclase and…
This volume provides a compilation of recent methods for studying protein phosphorylation.
This volume provides a methodology for the purification and characterization of the enzymes involved in phospholipid biosynthesis and for the generation of phospholipid biosynthetic mutants, and information on the mechanisms…
This volume aims to provide the reader with an introduction to data analysis in biochemistry, using examples from biochemical research. It is designed for use by those with little background…
Methods in Enzymology Volume 270 and its companion 271 cover the fundamentals and applications of liquid chromatography, electrophoresis, and mass spectrometry.
Volume 278 includes descriptions of the new methodologies and new developments in established fluorescence methods.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume…
This volume, and its companions - Methods of Enzymology volumes 330 and 334 - cover contemporary knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central…
Presents spectroscopic and physical methods for the determination of metal ion environments in metalloenzymes and metalloproteins. Major topics include spectroscopic methods for the determination of metal ion environments in metalloenzymes…
Methods in enzymology has previously devoted no volume to NMR. Volumes 176 and 177 rectify this omission. V.176 covered basic and advanced NMR techniques. This volume covers protein modifications for…
Provides methods for determining the metal content of metalloenzymes, metalloproteins, and other metallobiochemicals. It emphasizes the need for control of contamination, techniques for handling and dealing with contamination-sensitive molecules, and…
To understand the structures and dynamics of DNA and its interactions with ligans, complete analysis of its confirmational flexibility is required. This volume and Volume 212 of Methods in Enzymology…
Neoglycoconugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well.
Presents methods related to the role of anaerobic dissimilatory cycles and sulfur metabolism. This volume deals with topics such as: sulfate transport, dissimilatory sulfate metabolism, dehydrogenases, hydrogenases from sulfate-reducing bacteria…
This volume covers neoglycoconjugates. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as…
Oncogenes have become a central focus of cancer research. This volume may be used for developing protocols needed for their research.
Dealing with the field of biochemistry, this volume includes: Catalysts of Protein Folding - Protein Disulfide Isomerases, Cis-trans Peptidyl Prolyl Isomerases; Accessory Proteins - Chaperonins, Cochaperonins, Pap Proteins, Sec Proteins…
Suitable for researchers in various fields of life sciences, this work features prokaryotic and eukaryotic ABC transporters; nonmammalian ABC transport systems; mammalian P-Glycoproteins; multidrug resistance associated protein; cystic fibrosis transmembrane…
This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis…
Covers genetic information (cloning, gene expression) and information on human genetic diseases. This book includes topics such as: preparation of substrates and assay of enzymes; cloning, expression, and purification of…
This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the…
The contributions of this volume and its companion Volume 277 emphasize the revision and extension of statistical tools that underly all phases of structure determination, the practical and conceptual impact…
Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoglobin of artificial blood (blood substitute).
