Hands-On Experience of Microbial World: Microbiology Laboratory Exercises provides students with immersive experiences in microbiology. Organized into three comprehensive sections-general microbiology, health-related microbiology, and biotechnology utilizing microorganisms-the exercises cover a diverse range of topics. Students delve into essential techniques such as staining microorganisms for microscopic examination, mastering isolation and culturing methods, and studying microbial behavior. The book's final section explores advanced biotechnological applications, including the overexpression of proteins in E. coli and their subsequent isolation and purification using affinity chromatography techniques. Furthermore, students gain proficiency in visualizing the size and abundance of overexpressed proteins through SDS-PAGE analysis. Hands-On Experience of Microbial World is a dynamic and essential resource for courses and programs in microbiology.