This Safety Report provides guidance on the safe use of radiation for imaging and treatment in veterinary medicine with the objective of ensuring the safety and radiation protection of workers and members of the public. The publication addresses occupational exposure and public exposure in the use of radiation in veterinary medicine and safety issues that should be considered in order to be compliant with the International Basic Safety Standards (IAEA Safety Standards Series No. GSR Part 3). Consideration is given to the topics of source security and emergency response that might arise with the use of radioactive material in veterinary medicine. Although primarily intended for regulators and workers in veterinary medicine, the publication will also be relevant for professional bodies, ethics committees, and suppliers of equipment and software.