This publication provides guidance for monitoring and tracking carbon dioxide in the atmosphere or emissions from soil and plant materials using a laser carbon dioxide carbon isotope analyser. Through illustrated step-by-step standard operating procedures this publication assists scientists, technicians and students in techniques ranging from initial calibration of the instrument to collecting measurements and analysing data. When accurately utilized, this instrumentation can ensure proper evaluation of agricultural management practices to reduce soil carbon dioxide emissions and promote climate-smart agriculture. The final chapter of this publication also presents a case study that demonstrates how this instrumentation can be used.