This book presents the work carried out during the author's master's degree and relates the degradation of lignocellulose as it appears to the fungus. In the case of degradation studies, it is essential to understand the physiology of fungi so that production processes can be optimised. This book presents the main enzymes produced by the fungus Agaricus brasiliensis (Agaricus subrufescens) in two types of substrates, mapping the dynamics of enzyme production and the degradative counter-evidence of substrate elements using specific techniques. A must-read for anyone who works with mushroom cultivation and wants to deepen their knowledge.