The production and selection of somaclones in vitro for tolerant sugarcane cultivar involve a multifaceted process. It begins with the initiation of healthy explants, followed by callus induction and subsequent regeneration of shoots through tissue culture. The regenerated plantlets undergo a hardening phase before exposure to controlled drought and salinity stress conditions. Selection of plants exhibiting tolerance to these stresses is based on their performance under these conditions. Under induced drought stress SC1, SC2, SC3 of Isd 20, SC2, SC3 of Isd 35 and SC2, SC3 of Isd 37 and under induced salinity stress SC3 of Isd 28, SC3 of Isd 35 and SC2, SC3 of Isd 37 showed better performance in PVC pipe. Those somaclones also performed better at field condition and produced polymorphic bands when characterized with SCAR marker for selecting drought tolerant genotypes. This intricate process requires collaboration among experts in tissue culture, molecular biology, and plant breeding, ensuring a comprehensive approach to the development of resilient sugarcane varieties.