One of the key objectives of the biofuels world is to reduce the dependence on food resources, while increasing the use of new types of raw materials within advanced biofuels technologies. The most important biofuels that are now widely and commercially used in many countries are biodiesel and bioethanol. Bioethanol is a biofuel that can be produced by fermentation of sugary and starchy plants or by acidic hydrolysis of cellulosic sources. As a result, the use of biomass as raw material for biofuels should not create an alternate consumption area for human and animal food. On the other hand, indirect and direct energy consumption for the production of biomass, which will be used as biofuel raw materials, is also very important. Consumption of fossil fuels and other resources and damage to the environment must also be taken into account in biomass production, which will be used as raw materials for biofuels. This issue was the main basis for this work. Therefore, in this study, it is aimed to evaluate fossil fuel consumption and its associated CO2 emissions to cultivate sugar beet used as a biofuel raw material.