Digital pathology workflow implementation remains challenging. In laboratories worldwide, there are growing examples of successful implementation and pathologists acknowledging the advantages of this new model of practicing pathology.

This Special Issue of Diagnostics is a collection of records of successful digital pathology implementation for primary diagnosis or secondary applications, that serve as an inspiration to those readers who are still skeptical about it. These records highlight the advantages of the use of whole slide images such as sharing and the image analysis, as well as the virtues of the digital workflow per se. The holistic approach of the digital workflow comprehends practical interventions in the laboratory workstations that are demonstrated in this Special Issue, as well as their respective monitorization, quality control and impact on clinical practice.