Introduction to Biotechnology: Comprehensive overview of biotechnology, including its history, principles, and applications in various fields such as medicine, agriculture, and environmental science. CRISPR Technology: In-depth exploration of CRISPR-Cas9 technology, covering its discovery, mechanisms, and applications in genome editing, gene therapy, disease modeling, and beyond. Ethical and Regulatory Considerations: Discussion of the ethical implications of CRISPR technology, including concerns related to gene editing in humans, environmental risks, and social justice issues. Also, exploration of regulatory frameworks governing the use of CRISPR in different countries. Biotechnological Applications: Examination of biotechnological applications of CRISPR beyond genome editing, including CRISPR-based diagnostics, drug discovery, synthetic biology, and bioproduction of valuable compounds. Future Directions and Challenges: Analysis of emerging trends and future directions in biotechnology, with a focus on the potential of CRISPR technology to revolutionize healthcare, agriculture, and other industries. Also, discussion of challenges and limitations that need to be addressed for the responsible and beneficial use of CRISPR.