This handbook provides comprehensive coverage of the application of proteases in cancer therapy. Proteases make up to two percent of the human genome and play a critical role in the tumor microenvironment. The book delves into the applications of natural, synthetic, and non-coding RNAs in cancer therapy. It highlights how effective targeting of relevant proteases can help in cancer diagnosis and treatment. It covers the systems biology and bioinformatics approach in cancer drug development. The book is meant for researchers and professionals in cancer research, biochemistry, and physiology.