Peptide Nucleic Acids: Methods and Protocols

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Peter Eigil Nielsen has assembled a critically evaluated collection of key PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.