Biofuel Cells: The Design and Application of Biological Catalysts presents a detailed examination of biofuel cells, from their fundamentals and basic principles through to the latest technological, material, and bioengineering developments. The book follows a clear, step-by-step chapter structure that takes the reader through each stage of the design, construction, and operation of BFC-based devices. Chapters 1 and 2 provide a detailed review of the fundamentals and basic principles of microbial and biofuel cells, including the electrochemistry, materials and mechanics, and applications. Chapter 3 provides an in-depth examination of catalyst evolution, and Chapter 4 explains all aspects of electron transfer in enzymatic biofuel cells. Chapter 5 reviews all types of hybrid biofuel cells, including fabrication and design strategies for thermoelectric and triboelectric energy devices. In Chapter 6, advanced manufacturing techniques for biofuel cells and bio-devices are explained, including the working principles and methodologies for printing, microfluidics, fiber, microneedle, and others. Finally, Chapter 7 explores the diverse applications of biofuel cells and bio-devices, from biosensors and bioelectronics to capacitive biofuel cells. Chapters are supported by computational tools, working manuals for the techniques discussed, and detailed schematics and flowcharts for BCF fabrication. Biofuel Cells: The Design and Application of Biological Catalysts is an invaluable resource for graduate students and early career researchers interested in any aspect of biofuel cells and bio-devices and is specifically designed to benefit students from multiple backgrounds, including chemical engineering, electrical engineering, mechanical engineering, and biotechnology.